Efficacy of low-power laser irradiation in the prevention of D-galactose-induced senescence in human dermal fibroblasts

Abstract

Low-power laser (He-Ne) irradiation (LPLI) has been found to modulate various biological effects, especially those involved in promoting cell proliferation and metabolic regulation. However, the underlying mechanisms that LPLI prevents human cell senescence remain undefined. Herein, we devised a model enabling cell senescence using D-galactose for two days then treat with or without LPLI(< 15J/cm²), and investigated whether LPLI delays cell senescent in human dermal fibroblasts cells (HDF-a). First in this study, using SA-β-gal staining, compared with control cell we detected a lower frequency of SA-β-gal staining under the treatment of LPLI. Moreover, we found the growth rates of cell with LPLI was higher using CCK-8 analysis. Additionally, we also found LPLI induced HDF-a entered the irreversible G1 arrest measured by flow cytometry system. Therefore, LPLI may promote cell proliferation by stimulating cell-cycle progression and delay human cell senescence. Taken together, Low-power laser irradiation delay HDF-a cells senescence provides new information for the mechanisms of biological effects of LPLI.