EFFICACY OF JAVANESE TURMERIC ETHANOL EXTRACT IN ERADICATING STREPTOCOCCUS SANGUINIS AND PORPHYROMONAS GINGIVALIS BIOFILM

Rezon Yanuar,Widurini Djohan,Dewi Fatma Suniarti

doi:10.22159/ijap.2019.v11s1.155

Rezon Yanuar, Widurini Djohan + Show 1 more

Open Access

https://doi.org/10.22159/ijap.2019.v11s1.155

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Abstract

Objective: Javanese turmeric is an Indonesian native medicinal plant with antibacterial activities. This study aimed to analyze the efficacy of identifiedJavanese turmeric ethanol extract (IJTEE) in eradicating Streptococcus sanguinis and Porphyromonas gingivalis biofilms.Methods: Biofilm assay: Single and combination biofilms formed at different phases were exposed to IJTEE in 0.5–25% concentrations for 1 h. Thepercentage of eradication was tested using the microtetrazolium assay.Results: The efficacy of IJTEE in eradicating the biofilm was equal to that of chlorhexidine against the early phase of biofilm formation. IJTEE is moreeffective against S. sanguinis biofilm formation than against P. gingivalis biofilm formation.Conclusion: IJTEE can eradicate S. sanguinis and P. gingivalis biofilms.

Highlights

Chlorhexidine is an antiseptic mouthwash reported to reduce dental plaque formation and is considered as an antiseptic gold standard in maintaining oral health [1]
S. sanguinis and P. gingivalis were used at a bacterial concentration of 10−5
A study by Reina (2015) reported that the minimum inhibitory concentration (MIC) and MBC in turmeric ethanol extract identified against S. sanguinis were 5% and 15%, respectively, whereas those identified against P. gingivalis could not be established

Summary

Introduction

Chlorhexidine is an antiseptic mouthwash reported to reduce dental plaque formation and is considered as an antiseptic gold standard in maintaining oral health [1]. Chlorhexidine has some unexpected effects, such as staining on tooth surfaces, metal flavor, irritating the oral mucosa, and increased calculus formation [2]. These side effects have led to increased research on the development of other drugs, herbal medicinal plants, to determine an alternative antiseptic mouthwash that is efficacious, relatively safe, and developed using natural-based ingredients. Javanese turmeric (Curcuma xanthorrhiza Roxb.) is an original Indonesian herb that needs to be developed and utilized as herbal medicine owing to its health benefits [3]. Turmeric has been reported to contain antitumor, hypotriglyceride, anti-inflammatory, and hepatoprotection properties [4]

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Conclusion