Efficacy of Hyaluronic Acid in the Treatment of MIA-induced Ankle Osteoarthritis in Rats and its Effect on Antioxidant Response Element

Fatma M Halfaya,Ragab G H,Elkheir W A,Ahmed O M,Hagag U

doi:10.21608/jvmr.2020.34766.1020

Fatma M Halfaya, Ragab G H + Show 3 more

Open Access

https://doi.org/10.21608/jvmr.2020.34766.1020

Copy DOI

Abstract

Ankle osteoarthritis (OA) is a long-standing inflammatory degeneration disease; until now, its pathogenesis remains ambiguous. There is no complete remedy from OA and the present pharmacological therapy choices are restrained and combined with undesirable side effects. Clinically, Hyaluronic acid (HA) is widely consumed to cure OA. The present experiment aimed to assess the role of HA in the remedy of experimentally Monosodium iodoacetate (MIA) -induced ankle OA in the rat model. Thirty male Wistar rats were divided into 3 groups (each of 10 rats). Rats of group I were injected with 1 mg MIA in the right ankle joint for two successive days, while those of group II were treated with saline instead of MIA; and group III( osteoarthritic + HA) rats were injected with HA in the ankle joint at 2nd, 3rd, and 4th weeks following injection of MIA. Bodyweight, ankle measurement, total leukocytes count (TLC), antioxidant response element (ARE) level, and joint Magnetic Resonance Imaging (MRI) were investigated. HA reduced expressions of joint ARE level. HA also markedly reduced the TLC. The administration of HA decreases ankle measurement in MIA-induced OA rats. MRI of HA showed a gradual reduction in joint damage. These results suggest that HA has improvement effects on OA rats which are assessed through anti-inflammatory and antioxidant effects.

Highlights

Rats of group I were injected with 1 mg Monosodium iodoacetate (MIA) in the right ankle joint for two successive days, while those of group II were treated with saline instead of MIA; and group III( osteoarthritic + Hyaluronic acid (HA)) rats were injected with HA in the ankle joint at 2nd, 3rd, and 4th weeks following injection of MIA
These results suggest that HA has improvement effects on OA rats which are assessed through antiinflammatory and antioxidant effects
Once ROS overwhelms the capacity of the endogenous antioxidant, Keap 1 liberates NRF2 which move to the nucleus where it unites antioxidant response element (ARE) (Bhakkiyalakshmi et al 2018)

Summary

Target Gene ARE

Primer sequence Forward primer: 5’-TTGTAGATGACCATGAGTCGC-3’ Reverse primer: 5’-TGTCCTGCTGTATGCTGCTT-3’. Statistical analysis was achieved by using SPSS v.25. Results were expressed as mean ± standard error (SE), all statistical comparisons were performed by analysis of Duncan's test post hoc. The final body weight and body weight gain in normal control group, osteoarthritic group, and HA treated group are shown in Table (2) and Fig. The gains in body weight were comparable between MIA- rats from normal, and HA treated group at zero-days and six weeks the osteoarthritic rats gained less weight than the normal rats. The osteoarthritic control rats exhibited an obvious decrease in the body weight in the 6th week when compared to the normal control group. HA treated rats gained more weight when compared with the osteoarthritic control group. The treatment of the osteoarthritic rats with HA produced a noticeable increase of the bodyweight at the end of the experiment when compared to the osteoarthritic control rats

Normal group Osteoarthritic control group

Osteoarthritic control group

Zero day

There is no conflict of interest to declare