Abstract
Objective: This study is to assess the therapeutic effect of graphene oxide (GO) loaded with AWRK6 on endotoxin-induced sepsis.Method: AWRK6/GO was prepared by GO loaded AWRK6, with the structure characterization of AWRK6/GO conducted by atomic force microscope (AFM) and ultraviolet spectrophotometer, the sustained release rate of AWRK6/GO detected by high performance liquid chromatography (HPLC), and the neutralization ability of AWRK6/GO to lipopolysaccharide (LPS) tested by in vitro experiments. The levels of IL-8 and TNF-α in mouse cells after drug intervention were detected by ELISA; a LPS mouse model was established to observe the effects of drug intervention on the survival cycle and survival rate of mice.Results: The sustained drug release rate of AWRK6/GO reached 85% within 24 hours observed under in vitro conditions, with an efficient neutralization effect to LPS (P < 0.01); Compared with the control group, the intervention of LPS succeeded in remarkably elevating the levels of IL-8 and TNF-α in the whole blood and macrophages of the mice (P < 0.01), whose survival cycle and survival rate consequently observed an obvious decline (P < 0.01); The intervention with AWRK6 or AWRK6/GO predominantly brought down the levels of IL-8 and TNF-α in the whole blood and macrophages of mice given LPS (P < 0.01), resulting in an elevation of the survival rate and survival time (P < 0.01).Conclusion: GO loaded with cationic antimicrobial peptide AWRK6 exerts a rosy neutralization effect on endotoxin activity, with no obvious side effects on mice observed, which is of certain application value in the treatment of sepsis.
Highlights
To date, the treatment of sepsis, a disease of host immune disorders caused by bacterial infection, an array of therapeutic targets applied in clinical practice notwithstanding, still fails to yield an ideal outcome [1, 2]
To elevate the inhibition effect of AWRK6 on LPS, this study focuses on graphene nanoparticles loaded with AWRK6, and evaluates its effect in neutralizing endotoxin activity and treating sepsis, with an aim to provide a reference for the treatment of sepsis in the clinic
We identified the half-life of AWRK6/graphene oxide (GO) was 720 minutes
Summary
The treatment of sepsis, a disease of host immune disorders caused by bacterial infection, an array of therapeutic targets applied in clinical practice notwithstanding, still fails to yield an ideal outcome [1, 2]. The thinnest nanomaterial with only one atom thickness, is the basic unit of many nanomaterials [10]. It has been proven by in-depth research to have a wide range of applications in many fields such as medicine and chemical engineering [11]. To elevate the inhibition effect of AWRK6 on LPS, this study focuses on graphene nanoparticles loaded with AWRK6, and evaluates its effect in neutralizing endotoxin activity and treating sepsis, with an aim to provide a reference for the treatment of sepsis in the clinic
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
