Efficacy of Gen-Activated Osteoplastic Material Based on Octacalcium Phosphate and Plasmid DNA containing vegf Gene for Critical-sized Bone Defects Substitution

Abstract

Gene-activated osteoplastic materials are a principally new class of medical devices for bone plasty. The purpose of the study was to evaluate the biological action of the material based on octacalcium phosphate and plasmid DNA containing vascular endothelial growth factor (VEGF) gene. Study material was implanted into 10 mm diameter rabbit parietal bone defects. Formation of bone regenerates around material granules even in the center of the defect and the presence of bone marrow in intertrabecular space on day 60 after implantation was detected. In control group of animals - implantation of carrier without gene constructions - regeneration took place mainly on the bone defect margins. Use of gene-activated osteoplastic material resulted in pronounced osteoinduction that provided both acceleration of the regeneration and increase of new bone tissue formation intensity.