Efficacy of extraction methods for release of antioxidants in Caesalpinia bonducella seed pods

Abstract

Locally grown Caesalpinia bonducella seeds pod was subjected to ultrasonication and maceration for extraction by using different solvents like water, ethanol, and ethanol in combination with water at a different ratio to evaluate the extraction potential of phytoactive compounds. FTIR analysis was carried out for the quantitative estimation of functional groups in the different extracts. The highest yield was obtained in the ultrasonicated water extract is 21.9%. The utmost value of DPPH, ABTS, and SOSA inhibition activity was evaluated in the ultrasonicated seed pod aqueous extract 86.5%, 90.39%, 93.59%, respectively. The highest TPC found in ultrasonicated seedpod extracts was 21.91 mg GA/g. Aqueous extract of both extraction techniques had superior antioxidant activity and phenolic content which may have the potential for nutraceutical applications. The FTIR analysis showed the presence of alkanes, alkene, phenols, steroids, fatty acids, flavonoids, amino acids, etc. in all the ultrasonicated samples with higher antioxidant and phenolic content.