EFFICACY OF DIFFERENT IRRIGATING SYSTEMS WITH A NEW IRRIGATING SOLUTION IN REDUCING INTRACANAL ENTEROCOCCUS FAECALIS

Abstract

Aim: The aim of the present study was to evaluate the effect of QMixTM2in1in comparison with sodium hypochlorite irrigating solution on Enterococcus faecalis using different irrigation systems namely: Passive irrigation, ultrasonic activation, sonic activation. Materials and methods: 70 freshly extracted human single rooted teeth were collected. Root canals were prepared using Protaper Universal NiTi rotary file system in a crown down technique. Enterococcus Faecalis bacterial suspension in Trypticase soy broth was applied into the canal of each root. 65 Teeth were randomly divided into 3 experimental groups of 20 teeth each according to the technique used during irrigation protocol and a control group of 5 teeth without irrigation. Each group was subdivided into two sub-groups according to the type of irrigant. Samples were divided into four time intervals; S1 sample was taken immediately after infection of the teeth, S2 sample was taken immediately after irrigation, S3 sample was taken after 3 days incubation period after irrigation of the teeth, S4 sample was taken after 10 days from the infection. The method of evaluation of antimicrobial activity used was direct counting of colony forming units (CFUs) after irrigation. Results: there was increase in the bacterial count reduction with no statistical difference between QMixTM2in1 and 2.5% NaOCl after 3 days period from the irrigation procedures, while there was increase in the effect of QMixTM2in1 over 2.5% NaOCl after 10 days from irrigation procedures. While there wasn’t any difference found between the three agitation techniques on the antibacterial activity of both irrigants used. Conclusions: QMixTM2in1 irrigating solution proved antimicrobial efficacy in short and prolonged duration against Enterococcus faecalis. The antimicrobial efficiency of sodium hypochlorite can be improved using full concentration. It was also concluded that different agitation techniques have no effect on the antimicrobial efficacy.