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Abstract
Criteria were evaluated to identify levels of aggressiveness of the Dutch elm disease pathogen, Ophiostoma ulmi, and resistance in American elms. Twenty-year-old American elms were inoculated with putative nonaggressive strains PG442 and TN and aggressive strains PMP1, H961, and 16K. Strains PMP1 and H961 were confirmed to be aggressive and strains TN, PG442, and 16K to be nonaggressive. Six-month-old American elm seedlings were inoculated with the five O. ulmi strains, and aggressiveness was judged by disease symptoms and fungus multiplication. Vascular discoloration and numbers of colony-forming units most closely correlated with in vivo results. By most in vitro criteria, PG442 was categorized as aggressive
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