Efficacy of biocontrol agents against Fusarium oxysporum F. SP. Corianderi causing wilt of coriander under in vitro condition

Abstract

A laboratory experiment was conducted to find out the efficacy of different fungal and bacterial biocontrol agents against coriander wilt pathogen Fusarium oxysporum f. sp. corianderi. Among the seven antagonistic fungi tested using dual culture technique, Trichoderma harzianum JAU isolate 1 showed maximum reduced mycelial growth of Fusarium oxysporum f. sp. corianderi (46.23) than rest of antagonistic fungi. Whereas, the least effective biocontrol agent found was Trichoderma hamatum (NBA11 Tha 1) with 28.69 per cent mycelial growth inhibition of test fungus in vitro. The antagonistic actions of selected seven bacterial biocontrol agents were evaluated against the test fungus by dual culture technique (ring method). Among different bacterial antagonists, Pseudomonas fluorescens found significantly superior over rest of the biocontrol agents and showed maximum inhibition of mycelial growth of F. oxysporum f. sp. corianderi (99.76%). While, the least effective biocontrol agent found was Bacillus subtilis (KT894726) with 72.08 per cent mycelial growth inhibition of test fungus in vitro.