Abstract
Laboratory bioassays were carried out to determine the insecticidal efficacy of a Beauveria bassiana formulation (Bb38, Exosect Ltd, UK), which is based on the combined use of the fungus with the electrostatically charged dust Entostat. The efficacy of Bb38 was conducted on concrete surfaces against adults of the lesser grain borer, Rhyzopertha dominica, the rice weevil, Sitophilus oryzae, the confused flour beetle, Tribolium confusum and the rusty grain beetle, Cryptolestes ferrugineus, in two series of bioassays. In the first series, adults of the above species were exposed on concrete surfaces treated with Bb38 and mortality was recorded after 7 and 14 days of exposure (immediate mortality). These tests were carried out in all combinations of two temperatures, i.e. 25 and 30° C and two levels of relative humidity (r.h.), i.e. 55 and 75%. In the second series of bioassays, adults of the above species were exposed to Bb38 for 7 days, and after this interval, the surviving individuals were transferred in untreated concrete, where mortality was recorded again 7 and 14 days later (delayed mortality). From the species tested, R. dominica was by far the most susceptible, given that mortality reached 100% in almost all temperatures and r.h. levels. From the other species, S. oryzae and C. ferrugineus were moderately susceptible, while Bb38 was less effective for T. confusum. In the second series, immediate mortality for R. dominica was much higher than that of the other species. Moreover, delayed mortality of R. dominica reached 100% in contrast with the other species, where delayed mortality was low. Based on the results of the present work, we found that the insecticidal effect of Bb38 is not affected much by temperature and r.h., for the vast majority of the species and conditions tested. Moreover, in some cases, there was some post-exposure delayed activity of the formulation. This is one of the few studies that examine commercial formulations of fungal-based insecticides against stored product insects, which are expected to provide solutions in stored product protection, where traditional insecticides cannot be applied, as in the case of organic products.
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