Efficacy of Bacillus subtilis in Reducing Geotrichum candidum Toxicology in the White Rat Biological Systems

Abstract

The study aimed to evaluate the presence of Bacillus subtilis in the food dosed to laboratory rats to reduce the effect of G. candidum and its secretions of phenols on the rats’ health indicated by some chemical and biological blood variables. The experiment was conducted using 24 Rattus norvegicus adult male rats randomly divided into 8 groups 3 rats each. As treatments were; untreated control, dosing with 1 ml/Kg of Dimethyl Sulfoxide, pure orange juice, G. candidum infected juice, 1 ml B. subtilis+ 1 ml G. candidum infected juice, 60 mg/Kg fungal phenol, 1 ml B. subtilis+ 60 mg/Kg fungal phenol, or 1 ml B. subtilis. The results showed that dosing experimental rats with orange juice extracted from the orange parts infected with G. candidum, or dosing with phenols extracted from the fungus growth medium, led to a significant increase in blood variables including the numbers of WBC, GOT and GPT enzymes, and levels of blood urea. these two treatments also led to a significant decrease in hemoglobin and hematocrit levels, and blood content of protein. Whereas, the same treatments in the presence of B. subtilis maintained the values of all indicators under study within the normal limits close to control, and no negative effect was detected due to dosing with bacteria alone compared to the dosing with healthy juice.