Efficacy of Aspirin on Improvement of Peripheral Blood Circulation in Mice

Abstract

An in vivo model for assessing the efficacy of aspirin on the improvement of peripheral circulatory was developed by obtaining the cold-induced peripheral blood flow in mice using laser Doppler flowmetry (LDF) and temperature-controlled chambers. ICR mice (w=6) were placed in a temperature-controlled chamber and anesthetized with 1.5% isoflurane. LDF was used to measure the peripheral blood flow during whole-body warming for 10 min (37°C, baseline) and cooling for 40 min (until 13°C). Peripheral blood flow ratio showed significant difference between baseline and whole-body cooling after a lapse of 20 (0.92±0.20) and 40 min (0.33±0.13, p<0.001). Peripheral blood flows of mice administered aspirin (100 mg/kg) or control substance (saline) were measured. Mean peripheral flow ratio decrement of the hind foot was significantly higher in aspirin (0.23±0.10, p<0.05) than the control substance (0.15±0.04). These findings indicate that whole-body cooling is characterized by peripheral circulatory disturbance, which also influences the efficacy of aspirin in improving the peripheral blood circulation.