Abstract
AbstractPurposeBacteria colonization on the lid margin could play a crucial role in developing of biofilm causes of chronic infections such as blepharo‐conjunctivitis or lacrimal system disease. The aim of this study was to evaluate the efficacy of an ophthalmic fixed antibiotics/steroid combination (AC) containing tetracycline (TET), chloramphenicol (CAF) and colistin (CS), in the ratio 2:1:3, on bacterial planktonic growth and preformed biofilm.MethodsThe antimicrobial effect of AC against Staphylococcus aureus 815 and Pseudomonas aeruginosa 1 ocular strains was evaluated by time‐killing curves analysis at 30’, 2, 4, 6, 8 and 24 hr. The activity of AC (exposure of 2 and 6 hr) on preformed 24 hr‐biofilm was evaluated by cell viability and biomass measurements, expressed as logarithm of colony‐forming units/ml (Log10 CFU/ml) and optical density values, respectively.ResultsTime‐killing curves showed that AC was able to reduce the load of planktonic growth of both strains versus control. A significant reduction of 5 Log10 CFU/ml after 8h for S. aureus and after 4h for P. aeruginosa was reported. The AC was active against preformed biofilm, with a viability significant reduction (>2 Log10 CFU/ml versus control) of both strains at the exposure times. A biomass reduction of 40% and 60% for S. aureus and P. aeruginosa respectively, was detected at the exposure time of 2 hr and maintained at 6 hr.ConclusionsThe three antibiotics CAF, TET and CS present in AC showed a significant antimicrobial and antibiofilm effect. Antibiotics combination is a promising strategy for increasing treatment efficacy and for controlling resistance evolution. The results of this study, taken together, suggest the applications of this fixed combination in the treatment of chronic ocular infections where planktonic cells and biofilm are involved.Keywordsantibiotics combination, biofilm, ocular infection, lacrimal system
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