Abstract
In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants (Montanide ISA 61 VG (G1) and Alhydrogel®(G2)). The parameters of protection tested were fluke burden, faecal egg count and evaluation of hepatic lesions. In vaccinated group 1 we found a significant decrease in fluke burden in comparison to both unimmunised and infected control group (37.2%; p = 0.002) and to vaccinated group 2 (Alhydrogel®) (27.08%; p = 0.016). The lower fluke burden found in G1 was accompanied by a decrease in egg output of 28.71% in comparison with the infected control group. Additionally, gross hepatic lesions found in vaccine 1 group showed a significant decrease (p = 0.03) in comparison with unimmunised-infected group. The serological study showed the highest level for both IgG1 and IgG2 in animals from group 1. All these data support the hypothesis of protection found in vaccine 1 group.
Highlights
Fasciolosis caused by the liver fluke Fasciola hepatica is widespread worldwide with presence in more than 81 countries [1]
In this work we report the results of a vaccination trial performed in sheep using two different vaccine candidates, each one composed by a cocktail of F. hepatica recombinant molecules formulated in different adjuvants
During the last 30 years, the development of an effective vaccine against fasciolosis has been a challenging issue among researchers
Summary
Fasciolosis caused by the liver fluke Fasciola hepatica is widespread worldwide with presence in more than 81 countries [1]. Fasciolosis supposes a major problem in farming industry since it is of importance in livestock, specially ruminants in which the disease is responsible for substantial economic losses estimated at 3.2 US$ billion/year [3]. These costs are due to both losses in production (i.e. milk, carcass composition as well as a delay to reach an appropriate slaughter weight) and treatment with anthelmintic drugs [4,5,6]. There is a growing concern in consumers about the presence of chemical residues in animal products and the presence of these residues often force farmers to have withdrawal periods before animal products could be consumed
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