Efferent synaptic organization of the olivary pretectal nucleus in the albino rat. An ultrastructural tracing study.

Abstract

In this study an ultrastructural analysis was made of the efferent projections of the olivary pretectal nucleus in the rat. The anterograde tracer Phaseolus vulgaris leucoagglutinin was injected iontophoretically into the olivary pretectal nucleus. Ascending and descending pathways were studied. In the descending pathway special attention was paid to the fine structural features of the olivary pretectal nucleus efferents projecting to the Edinger-Westphal nucleus, the interstitial nucleus of Cajal, the nucleus of Darkschewitsch and the periaqueductal gray. The projection to the superior colliculus and the pontine nucleus was also studied at the ultrastructural level. All the labeled terminals in the descending pathway showed ultrastructurally similar features: clear, round vesicles and electron dense mitochondria. The terminals made asymmetric synaptic membrane specializations (Gray type I), the postsynaptic profiles were dendritic. In the interstitial nucleus of Cajal and the superior colliculus the terminals are organized in glomerulus-like structures. The terminals in the descending pathway were enwrapped by astrocytic processes, also in the glomerulus-like structures. In the ascending pathway the projection to the ventral part of the lateral geniculate nucleus was studied. Almost all terminals in the ascending pathway showed similar ultrastructural features as in the descending pathway: electron dense mitochondria, clear, round vesicles and asymmetric synaptic membrane specializations (Gray type I). The terminals are organized in glomerulus-like structures. To identify the projecting neurons in the interstitial nucleus of Cajal and the Edinger-Westphal nucleus, retrograde tracing experiments were performed. Therefore the beta subunit of cholera toxin conjugated with horseradish peroxidase was injected into the facial nucleus.(ABSTRACT TRUNCATED AT 250 WORDS)