Effects or N, N'-di-(m-methylphenyi) -3,6-dimethyl-l, 4-dlhydro-l ,2,4,5-tetrazine-l, 4-dicarboam-ide on proliferation and apoptosis of A549, EBC-1, MA cells in vitro

Abstract

Objective To study the effect of N, N-di-( m-methylphenyi) -3,6-dimethyl-l ,4-di-hydro-1,2,4,5-tetrazine-1,4-dicarboamide (ZGDHu-1) on proliferation and apoptosis of A549, EBC-1 and MA cells in vitro. Methods Different concentrations of ZGDHu-1 were incubated with A549, EBC-1, or MA cells for different durations. The proliferation inhibition was analyzed by alive cell count, Methyl thi-azolyl tetrazolium (MTT) assay and BrdU-ELISA. Cell apoptosis was measured by cell morphology, DNA content, Annexin-V/PI and Hoechst33258 labeling method. Results ZGDHu-1 could inhibit proliferation of A549, EBC-1 and MA cells within a certain range of treating duration and does, with a 48 h IC50 of 165, 106, 210μLg/L, 72 h of 42, 18, 90μg/L, and a majority of A549, EBC-1 and MA cells were arrested in G2/M phase. The apoptosis of A549, EBC-1 and MA cells was confirmed by type cell morphology, DNA fragment, sub-G1 phase and Hoechst33258 and Annexin/PI labeling method. Conclusion ZGDHu-1 can inhibit proliferation and induce apoptosis of A549, EBC-1, and MA cells. Key words: ZGDHu-1; Lung carcinoma; Proliferation; Apoptosis