Effects on chrysin on lipid and xenobiotic metabolizing enzymes in l-NAME-induced hypertension

Abstract

Objective: N ω -nitro-l-arginine methyl ester (l-NAME) is a non-specific nitric oxide (NO) synthase inhibitor, commonly used for the induction of NO-deficient hypertension. Hypertension is a significant risk factor in cardiovascular complications. Materials and Methods: This study was undertaken to investigate the effects of chrysin on lipid metabolizing enzymes, xenobiotic metabolizing enzymes, and microalbuminuria and N -acetyl-β-d-glucosaminidase (NAG) in urine in l-NAME-induced hypertensive rats. Hypertension was induced in adult male Wistar rats weighing 180-220 g by oral administration of l-NAME (40 mg/kg BW) in drinking water for 4 weeks. Rats were treated with chrysin (25 mg/kg BW) for 4 weeks. Results: The activity of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase increased significantly in the liver and kidney, whereas the activities of lipoprotein lipase and lecithin cholesterol acyl transferase decreased significantly in the plasma of hypertensive rats. Conclusions: The xenobiotic phase I enzymes, microalbuminuria, and NAG significantly increased, whereas xenobiotic phase II enzymes decreased in l-NAME-treated rats. Oral administration of chrysin reduced hyperlipidemia-related risk of hypertension.