Abstract
Objective To explore the changes of zinc and ubiquitin conjugation in neurons following traumatic brain injury (TBI) and to investigate the mechanism of zinc on secondary neuronal injury after TBI.Methods The weight drop-induced TBI model was established.The changes of zinc and ubiquitin conjugation in neurons were detected by ZP4 fluorescence staining and western blotting,respectively.The survival of neurons was detected by nissle staining.Eventually,we studied the changes of zinc and ubiquitin conjugation in the neurons of human pericontusinal using the AMG staining and and western blotting,respectively.Results Zinc accumulation and ubiquitin conjugation and ultimately neurodegeneration were found in hippocampal neurons following traumatic brain injury by ZP4 fluorescence staining,western blotting,and nissle staining.Scavenging Zn2 + reduced TBI-induced ubiquitin conjugation and protected neurons from TBI insult.Lastly,in human brain cortical neurons,we detected zinc accumulation and increased ubiquitin conjugated protein following brain trauma using AMG staining and western blotting.Conclusion All these findings indicate that alterations in Zn2 + homeostasis impair the protein degradation pathway and ultimately induce neuron-injuries following traumatic brain injury. Key words: Traumatic brain injury; Neuron; Ubiquitin ; Zinc
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
