Effects of zinc bearing palygorskite supplementation on the growth performance, hepatic mineral content, and antioxidant status of broilers at early age

Weili Yang,Chao Wen,Yefei Cheng,Yanmin Zhou,Yueping Chen

doi:10.5713/ajas.16.0551

Weili Yang, Chao Wen + Show 3 more

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https://doi.org/10.5713/ajas.16.0551

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Abstract

ObjectiveThis study was conducted to investigate effects of zinc (Zn) bearing palygorskite (ZnPal) supplementation on growth performance, hepatic mineral content, and antioxidant status of broilers at early age.MethodsA total of 240 1-day-old Arbor Acres broiler chicks were allocated into 5 treatments with 6 replicates of 8 chicks each. Birds in 5 treatments were fed a basal diet supplemented with 0 (Control group; Analyzed Zn content: 81 mg/kg), 20, 40, 60, and 80 mg/kg Zn as ZnPal for 21 days, respectively. Blood, liver and intestinal mucosa were collected at 21 days of age.ResultsTreatments did not affect growth performance of broilers during the 21-day study (p> 0.05). The contents of hepatic Zn and magnesium (Mg) were linearly increased (p<0.001) by ZnPal supplementation. ZnPal inclusion linearly (p = 0.007) reduced malondialdehyde (MDA) concentration in serum. The activity of total superoxide dismutase (T-SOD) in liver increased linearly (p = 0.001) with concentration of ZnPal in diet. ZnPal inclusion linearly (p = 0.036) and quadratically (p = 0.005) increased T-SOD activity, and linearly (p = 0.012) increased copper/zinc superoxide dismutase (Cu/Zn SOD) activity in jejunal mucosa. The maximum responses of hepatic and jejunal antioxidant enzymes activities (T-SOD and Cu/Zn SOD) were found when supplementing the basal diet with 60 mg/kg Zn as ZnPal. Furthermore, ZnPal supplementation quadratically (p = 0.001) increased Cu/Zn SOD activity in ileal mucosa, and its maximum activity was observed in the diet supplemented with 20 mg/kg Zn as ZnPal.ConclusionZnPal supplementation did not alter growth performance of broilers. Dietary ZnPal inclusion could increase concentrations of hepatic trace minerals (Zn and Mg) and inhibit lipid peroxidation by reducing serum MDA accumulation, with the optimal dosage of Zn from ZnPal being 80 mg/kg diet (analyzed Zn content in the diet: 165 mg/kg), and 60 mg/kg Zn as ZnPal (analyzed Zn content in the diet: 148 mg/kg) was the optimum dosage for broilers to achieve maximum antioxidant enzyme activities.

Highlights

The reactive free radicals, like reactive oxygen species (ROS), are routinely produced during meta bolism in animals
Hepatic antioxidant status Zn bearing Pal (ZnPal) supplementation (Table 6) linearly (p = 0.001) increased the activity of total superoxide dismutase (T-SOD) in the liver of broilers, and its maximum response was observed in the diet supplemented with 60 mg/kg Zn as ZnPal (p
Bun et al [5] showed that the incorporation of an organic Zn source to a corn–soybean basal diet (29.6 mg Zn/kg diet) from 0 to 60 mg/kg did not alter weight gain, feed intake and feed/gain ratio of healthy birds or birds challenged with Eimeria tenella

Summary

Introduction

The reactive free radicals, like reactive oxygen species (ROS), are routinely produced during meta bolism in animals. The amounts of these radicals maintain a balanced status and are not harmful for metabolism and health of animals in the presence of an effective antioxidant defense system [1]. When the over production of ROS exceeds the defence capacity of the host’s antioxidant system, it will trigger lipid peroxidation that would induce oxidative damages to DNA and proteins, disintegrate the cellular membrane, disturb the balance between oxidants and antioxidants, and eventually induce the occurrence of oxidative stress, which is often regarded as one of the causes of several pathologies that affect poultry growth [1,2].

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