Effects of Yam Polysaccharides on P-selectin expression and macrophage infiltration in diabetic nephropathy model rats

Abstract

Objective: Observe effects of Yam polysaccharides on P-selectin (Ps) expression and macrophage infiltration in diabetic nephropathy model rats. Method: Establish a Streptozocin-induced diabetic nephropathy rat model. After successful modeling, the rats are assigned into one of the following groups (10 rats/group): Model Group, Yam Polysaccharides Groups (50, 100, 200 mg·kg -1 ), and Metformin Hydrochloride Tablets (800 mg·kg -1 ) Group. The rats in the Yam Polysaccharides Dose Groups are administered intragastrically, and the rats in the Normal Control Group and the Model Group are administered intragastrically with an equal volume of distilled water, qd, for 8 consecutive weeks. Kidney weights, kidney indexes, 24-h urine protein excretion, blood glucose (Glu), creatinine (Crea), urea nitrogen (BUN), and albumin (ALB) in rats, as well as nitric oxide (NO), malonaldehyde (MDA), and superoxide dismutase (SOD) in kidney homogenate are measured. Changes in the expressions of glomerular Ps, CD68, transforming growth factor β1 (TGF-β1) in rats are observed by immunohistochemistry. Results: Blood glucose and 24-h urine protein excretion