Abstract
Phospholipids, tagged with P32, of liver and spleen of normal and irradiated mice were separated by silicic acid column chromatography. The speciiic radioactivity of the total phospholipids and of seven groups of phosphatides was determined at various times after the injection of the P32 in both normal and irradiated animals. The uptake of P32 into the total liver phospholipids was higher in the irradiated animals than in the controls. This was due to increased specific activity of the cephalins and lecithins. The specific activity of inositides and lysolecithin was not greatly changed by irradiation, whereas that of the sphingomyelins was increased at 2 and 4 hours but decreased after 8 hours. The specific radioactivity of liver phospholipids reached a maximum at 8 hours after injection of the P32 in normal animals but occurred at 6 hours in the irradiated animals.
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