Effects of x-irradiation on lipide metabolism. II. Uptake of P 32 into rat liver phospholipides

Abstract

1. 1. The effect of whole-body x-irradiation on the incorporation of inorganic P 32 into phospholipides of rat liver and liver cell fractions were studied both in vitro and in vivo. 2. 2. Liver slices from rats, 24 hr. after exposure to 1000 or 2500 r., incorporated more P 32 into phospholipides than did slices from unirradiated rats. Increased incorporation also occurred in the various cell fractions studied, namely nuclei, mitochondria, microsomes, and supernatant. 3. 3. No increase in P 32 incorporation into phospholipides of the liver or liver cell fractions was noted in rats which were injected with inorganic P 32 24 hr. after irradiation with 2500 r. 4. 4. The significance of these results is discussed and possible explanations are proposed for the differences between the in vitro and in vivo findings.