Abstract
Incorporation of 14C-pyruvate into the citric acid cycle metabolites was measured in rat thymocytes X-irradiated in vitro. X-irradiation caused no significant changes in 14C-pyruvate incorporation into the metabolites separated by Dowex-1 formate column chromatography. Glycogen content of the cells decreased, whereas malate showed a significant accumulation after irradiation. From the results, the previously reported increase in labeling of citric acid cycle metabolites derived from 14C-glucose was explained as the result of the enhanced glycolysis in the thymocytes after irradiation.
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