Effects of Wuweizi syrup on brain aging mice model induced by d-galactose

Abstract

ObjectiveTo observe the effect of Wuweizi syrup on brain aging mice model induced by d-galactose in mice. Methods72 mice were randomly divided into blank group, model group, naokangling group and low, middle and high dose Wuweizi syrup group. After 3 days of adaptive feeding, except for the blank group, the rest mice were subcutaneously (sc) injected with d-galactose 1.25 g/kg at neck back to replicate the aging model. Sc injection was given for 40 consecutive days, and corresponding drugs were given by gavage on the 11th day of modeling. On the 39th day, the dark experiment was carried out to measure the learning ability of mice. After the last administration for 2 h, brain, liver, thymus and spleen were taken and weighed to calculate the organ index. Blood was taken and CAT, GSH and SOD levels in whole blood were determined. Some brain and liver tissues were taken to prepare homogenates, and MDA levels in tissue homogenates and plasma were measured. Brain, liver, thymus and spleen were stained with HE, and pathological sections were made for observation under light microscope. ResultsCompared with the blank group, in the model group, the incubation period of mice was significantly reduced and the number of shuttle back and forth was significantly increased (P < 0.01); Spleen and brain indexes decreased significantly (P < 0.05), thymus indexes were decreased significantly, and liver indexes were increased significantly (P < 0.01); The levels of CAT, GSH and SOD in whole blood were significantly decreased (P < 0.01); MDA levels in liver homogenates, brain homogenates and plasma were significantly increased (P < 0.01); Pathological changes of brain, liver, thymus and spleen were observed under microscope. Compared with the model group, naokangling group and low, middle and high dose Wuweizi syrup group can significantly improve the incubation period of mice and reduce the number of shuttle back and forth (P < 0.01), can decrease or increase the organ index with different degree (P < 0.01 or P < 0.05), can significantly increase the levels of CAT, GSH and SOD in whole blood (P < 0.01), and significantly reduce the levels of MDA in plasma, liver homogenate and brain homogenate (P < 0.01), as well as can improve pathological changes of brain, liver, thymus and spleen with different degree. ConclusionThe mice aging model was successfully replicated, and Wuweizi syrup can significantly improve the memory, biochemical indexes and pathological status of brain aging model mice.