Effects of white clover (Trifolium repens L.) on plant and soil nitrogen and soil organic matter in mixtures with perennial ryegrass (Lolium perenne L.).

Abstract

To increase our insight into the above- and belowground N flows in grass and grass-clover swards relations between crop and soil parameters were studied in a cutting trial with perennial ryegrass (Lolium perenne) monocultures and ryegrass-white clover (Trifolium repens) mixtures. The effects of clover cultivar on herbage yield, the amount of clover-derived nitrogen, apparent N transfer to companion grass, dynamics of N and organic matter in the soil were estimated. The grass monocultures had very low DM yields (<2.1 t ha[-1]) and a low N concentration in the harvested herbage. During 1992-1995 the annual herbage DM yield in the mixtures ranged from 7.0 to 14.3 t ha[-1], the white clover DM yield from 2.4 to 11.2 t ha[-1] and the mean annual clover content in the herbage DM harvested from 34 to 78%. Mixtures with the large-leaved clover cv. Alice yielded significantly more herbage and clover DM and had a higher clover content than mixtures with small/medium-leaved cvs. Gwenda and Retor. Grass cultivar did not consistently affect yield, botanical composition or soil characteristics. The apparent N[2] fixation was very high, ranging from 150 to 545 kg N ha[-1] in the different mixtures. For each tonne of clover DM in the harvested herbage 49 to 63 kg N was harvested, while the apparent N transfer from clover to grass varied between 55 and 113 kg N ha[-1] year[-1]. The net N mineralization rate was lower under monocultures than under mixtures. The C mineralization and the amounts of C and N in active soil organic matter fractions were similar for monocultures and mixtures, but the C:N ratio of the active soil organic matter fractions were higher under grass than under mixtures. This explains the lower N mineralization under grass.