Abstract
Objective To investigate the effects of the water-soluble extract from cactus pear fruit (CPFWE) on differentiation of obsteoblasts. Methods Rat osteoblasts were isolated as an in vitro model. MTT assay was used to evaluate the growth and proliferation of rat osteoblasts. The spectrophotometer was used to determine alkaline phosphatase (ALP) activity and hydroxyproline (Hyp) content. By alizarin red staining, bone nodule formation was observed. Results In the middle stage of differentiation, CPFWE observably stimulated ALP activity and Hyp content in osteoblasts. Especially, CPFWE increased the ALP activity by (57.748±4.071)% at the concentration of 50.000 μg/ml for 9 days and Hyp content by (26.444±0.990)% at the concentration of 25.000 μg/ml for 22 days as compared with the control group. In the later stage, the CPFWE significantly increased the area of nodules by (43.840±1.200)% at 50.000 μg/ml concentration and increased IOD by (48.241±1.760)% at 6.250 μg/ml for 18 days. Conclusion The effects of CPFWE on bone formation by rat osteoblasts suggest that these phenolics may have beneficial effects on bone health. Key words: Cactus pear fruit; Water-soluble extract; Osteoblast cell; Bone formation
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