Effects of water stress on N2 fixation and grain yield of Phaseolus vulgaris L.

Abstract

Most dry bean production in Mexico is under non-irrigated conditions in the semi-arid highlands. One of the most limiting factors is insufficient moisture during the reproductive stage and sometimes during the vegetative stage. The objective of this experiment was to study the effect of drought on nodulation, N2 fixation and grain yield of beans. The cultivars evaluated were: Flor de Mayo Bajio, Bayocel, Bat-477 and Honduras-35. The treatments were water stress treatments during vegetative or reproductive stage and a control of minimal stress. To measure N2 fixation, 15N-labelled fertilizer was used. Data for soil moisture, nodule number and nodule dry weight, as well as, straw and grain yield and total N were taken. The results indicated that water stress during vegetative stage depressed nodulation temporarily, but after watering regularly plants not only recovered completely but were nodulated better than the control. Water stress during the reproductive stage depressed nodulation and after watering there was no recovery. Grain yield was not affected by water stress during vegetative stage but it was reduced when water stress was imposed during the reproductive stage. The percentage of N derived from fixation varied among cultivars but was not affected by water stress treatment. The highest N2 fixation occurred in Bayocel and Bat-477 and the lowest in Honduras-35 and F.M. Bajio, although the amounts were not as low as in some other reports.