Effects of Vitamin C and Squid Oil Supplementation on Gonad Maturation of Climbing Perch Broodstock (Anabas testudineus Bloch).

Abstract

This study aims to determine the effectiveness of adding vitamin C and squid oil to commercial feed for broodstock fish (Anabas testudineus Bloch) on the speed of gonadal maturation and growth. The study was conducted for three months using a completely randomized design with 4 treatments and 3 replications. The test fish were 10 each in each treatment hapa, the treatment of feed A (Commercial feed =CF); B (CF + Vit c 2gr/kg; C (CF+ squid oil 3 ml/kg) and D (CF+ Vitamin C 2 g/kg + Squid oil 3 ml/kg). Feed was given in the morning and evening satiation for 30 days. The results showed that the commercial feed added with a combination of vitamin C 2000mg/kg feed and squid oil as much as 3mg/kg feed (Treatment D) gave the best gonadal maturity level. This is indicated by the average Gonadosomatic Index (GSI) of 4.20% and the average HepatoSomatic Index (HSI) of 3.91%, higher than other treatments. Supported by statistical test results showed very significant differences in somatic gonadal index, hepatosomatic index, egg diameter and absolute fecundity. Meanwhile, growth and condition factors did not show significant differences. There is a tendency that if the dose of vitamin C and squid oil is increased again, it will be able to increase the gonad maturity of the broodstock of climbing perch.