Abstract
The specific activities of three B12-dependent enzymes in Rhizobium meliloti were compared in relation to morphological changes in the bacterium. The critical levels of ribonucleotide reductase and methylmalonyl-CoA mutase seemed to be maintained, while the level of methionine synthase was probably insufficient for cell multiplication. A close relationship was observed between the methionine synthase level and morphological changes in the bacterium. The addition of folate with methionine to the cobalt-deficient medium did not have a positive effect on bacterial growth. A sufficient amount of tetrahydrofolate was detected in the cobalt-deficient elongated cells. These findings might suggest the important role of methionine synthase in cell multiplication, which was unpredictable from the methylfolate trap hypothesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
