Effects of Vegetation Restoration on Soil Bacterial Communities, Enzyme Activities, and Nutrients of Reconstructed Soil in a Mining Area on the Loess Plateau, China

Mingde Hao,Yongxing Cui,Shilei Zhu,Yanjiang Zhang,Pengfei Li,Xingchang Zhang

doi:10.3390/su11082295

Abstract

Soil microbes are the main driving forces and influencing factors of biochemical reactions in the environment. Study of ecological recovery after mining activities has prompted wider recognition of the importance of microbial diversity to ecosystem recovery; however, the response of soil bacterial communities to vegetation restoration types and soil biochemical properties remains poorly understood. The purpose of this research was to explore the soil bacterial communities and soil biochemical properties at four sampling sites (brushland (BL), forestland (FL), grassland (GL) and unreclaimed land (UL)) on the Loess Plateau, China, to evaluate the effect of vegetation restoration on the reconstructed soil in mining areas. In August 2017, samples were collected at the Heidaigou coal mine dumps. Illumina MiSeq sequencing was used to identify the structure of the soil bacterial community and evaluate its relationships with soil biochemical properties. The results showed that soil biochemical properties (soil organic matter, available phosphorus, urease, sucrase, microbial biomass carbon and microbial biomass nitrogen) were significantly increased in BL, FL and GL relative to UL, indicating that the soil quality was significantly improved by vegetation restoration. In addition, the results showed that the vegetation restoration on the reconstructed soil in the mining area could significantly improve the operational taxonomic units (OTUs), abundance (ACE and Chao1) and diversity (Shannon and Simpson) indices of bacterial community and the dominant phyla were Proteobacteria, Actinobacteria and Acidobacteria. With vegetation restoration, the relative abundance of Proteobacteria and Acidobacteria showed an increasing trend, while that of Actinobacteria showed a decreasing trend, and the dominant phyla were only significantly correlated with a few biochemical properties. Moreover, there were no changes in soil bacterial community structures across the four sampling sites and the response of the bacterial community to biochemical properties was not obvious. This implies that, although the region has experienced about 20 years of vegetation restoration, the microbial community still maintains good stability and lagging response to soil biochemical properties. Since the BL soil had better biochemical properties and higher bacterial richness and diversity, it was recommended as the optimum vegetation restoration type for soil reclamation in this area.

Highlights

Mining activities causes extensive damage to soil and changes the soil environment, adversely altering soil properties and the function and stability of microbial community structure [1]
These findings indicate that vegetation restoration significantly iSmusptarinoavbielidtyt2h0e19b, a11c,txerFiOaRl cPoEmERmRuEnViItEyWrichness and diversity compared with the unreclaimed land (UL)
We found that major bacterial phyla, such as Proteobacteria, Acidobacteria and Gemmatimonadetes, were spread in Quadrants II and III, except for Actinobacteria which spread in Quadrant I

Summary

Introduction

Mining activities causes extensive damage to soil and changes the soil environment, adversely altering soil properties and the function and stability of microbial community structure [1]. The final objective of ecological restoration and reclamation in mining areas is to restore productivity on the post-mining land and maintain the sustainable development of ecosystem [3,4]. Due to the important mediating role of soil microbes in ecosystems, the restoration of the soil microbial community is a key process of soil restoration and plays a positive role in achieving soil health and sustainable utilization [4]. Studies have shown that soil microbes play a major role in regulating a variety of ecological functions [7,8]. Microbes obtain nutrients and habitats from the soil and have an impact on soil quality [9]

Objectives

Methods

Results

Conclusion