Abstract
Crinum asiaticum Linn. has been used in Thai traditional medicine to relieve inflammatory symptoms and treat osteoarthritis. There have been reports on its potent anti-inflammatory property but nothing on the effects of different pretreatments on its chemical properties and anti-inflammatory activity. Pretreatment of herbal raw materials is an important step which affects the overall quality of Thai traditional medicine. The objectives of this study were to investigate different treatments of C. asiaticum leaves prior to ethanolic extraction and to compare the extracts for their anti-inflammatory activity and chemical properties. The treatments included hot air drying in an oven, microwave drying, traditional grilling on a charcoal stove before drying in an oven, and temperature shock in hot and cold water before hot air drying. The anti-inflammatory activity and chemical properties of the extracts were analyzed using the established methods. Results showed that 95% ethanolic extract of hot air oven-dried leaves had the highest anti-inflammatory activity and total phenolic and lycorine contents. We recommend hot air drying as a preextraction treatment for C. asiaticum leaves for its simplicity, best retention of the herbal quality, and suitability for scaling up to an industrial process.
Highlights
Inflammation is a primary physiologic response of tissue to pathogens, cell injury, and damaged tissues
Yields (%) of the ethanolic extracts of C. asiaticum leaves obtained from different preextraction treatments are shown in Table 1. e highest yield was obtained from the grilling method (CAG; 25.75%)
Our study showed that lycorine exerted strong anti-inflammatory activity by inhibiting nitric oxide (NO) production, but it only slightly inhibited TNF-α and prostaglandin E2 (PGE2) production in RAW264.7 cells
Summary
Inflammation is a primary physiologic response of tissue to pathogens, cell injury, and damaged tissues. Proinflammatory cytokines, inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-alpha (TNF-α) produced during the inflammatory process cause damage of cell, tissue, and organ [1, 2]. E inflammatory mediators such as nitric oxide, IL-1, TNF-α, and COX-2 contribute to cartilage and joint degeneration [3]. Nonsteroidal anti-inflammatory drugs (NSAIDs) are the common treatment for OA pain and inflammation. (Amaryllidaceae family) has long been used in Southeast Asian and ai traditional medicine to relieve pain and as a treatment for inflammatory diseases. Southeast Asian countries use C. asiaticum for treatments of wounds, swellings, pain injuries, and inflamed joints, and as an antidote for poisons or toxins [5,6,7]. C. asiaticum leaf is a plant component in Phra-aung-kob-phra-sean remedy commonly used to treat
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
