Effects of Various Glucogenic Sources on Production and Metabolic Responses of Dairy Cows Fed Grass Silage-Based Diets

Abstract

Four rumen cannulated Finnish Ayrshire cows in midlactation were used in an experiment designed as a 4×5 incomplete Latin square with 2-wk periods to compare effects of glucogenic substrates on grass silage-based diets. The five treatments were continuous infusions of 1) water (control), 2) casein 300g/d, 3) glucose 300g/d, 4) propionic acid 247g/d, and 5) barley starch 270g/d. Substrates were infused either into the rumen (propionic acid) or into the abomasum (other substrates). As a basal diet, cows were fed a formic acid treated grass silage ad libitum (digestible organic matter 690g/kg dry matter [DM], crude protein [CP] 131g/kg DM) and a barley-rapeseed concentrate (CP 141g/kg DM) at a rate of 7kg/d. Production responses to glucogenic substrates other than casein were negligible, suggesting that glucose supply of the cows did not primarily limit milk production. However, with casein cows produced significantly more milk, milk protein, and lactose than with other glucogenic substrates. Casein increased urea and essential amino acid (EAA), and decreased nonessential AA (NEAA) in arterial plasma compared with other substrates, suggesting that casein provided precursors both in terms of NEAA for gluconeogenesis and EAA for milk protein synthesis. This puts forward that providing the AA needs of the mammary gland for milk protein synthesis are met, glucose supply may become the next limiting factor for milk protein synthesis in cows fed diets based on restrictively fermented grass silage. The limited supply of AA from the basal diet, and possibly the low production levels of cows partly invalidated the hypothesis of monitoring differing glucogenic substrates for grass silage-based diets.