Effects of valsartan on p-selectin in myocardial ischemia reperfusion injury of rat

Abstract

Objective To observe the effects of angiotensin Ⅱ type 1 receptor blocker, valsartan on the release of P-selectin in rat model of myocardial ischemia reperfusion injury, and the preliminary action mechanism of valsartan. Methods Ninety wister rats were randomly divided into four groups, Group Ⅰ ( Sham operated group, n = 10), Group Ⅱ (Iscbemic group, n = 20), Group Ⅲ (Ischemic reperfusion Group, n =30) and Group Ⅳ (Valsartan group, n =30). Group Ⅳ was given valsartan by direct gastric garages 20mg/(kg·d). Group Ⅰ,Ⅱ, Ⅲ were given normal saline garages 2ml/d). After four weeks of pretreatment, the middle point of left anterior coronary artery (LAD) was sham ligated for 30 minute( Group Ⅰ ). The middle point of left anterior coronary artery (LAD) was ligated for 30 minutes( n = 10) and 60 min ( n = 10) in Group Ⅱ. The middle point of left anterior coronary artery (LAD) was ligated for30 minutes, followed by 60 minutes ( n = 10), 120 minutes( n = 10), 360minutes reperfusion( n = 10) in Group Ⅲ. The middle point of left anterior coronary artery (LAD) was ligated for 30 minutes, followed by 60 minutes( n = 10), 120 minutes( n = 10), 360minutes reperfusion ( n =10) in Group Ⅳ. Blood samples were taken from right atrium for measurement. The contents of p-selectin were detected by the method of Enzyme-Linked Immunosorbnent Assay (ELISA). Results The expression of P-selectin continually elevated after reperfusion( P < 0.05 or P <0. 01). P-selectin level in group Ⅲ was higher than that in group Ⅳ( P <0. 01 ). The valsartan could reduce the release of p-selectin.Conclusions Valsartan could relieve myocardial ischemia reperfusion injury of rat, which may be through reducing p-seleetin of plasma. Key words: Valine/AA/PD; Myocardial reperfusion injury/PC/ME; P-selectin/DE/ME