Effects of vagotomy on pathophysiology in dog's acute necrotizing pancreatitis model

Abstract

Objective To study the effect of cutting vagus nerves on pathophysiology in dog model of acute necrotizing pancreatitis (ANP).Methods Twenty-six male adult hybrid dogs with good health were randomly divided into sham operation group (SG),control group (CG) and vagotomy group (VG).ANP model was induced by the retrograde injection of 5％ sodium taurocholate and trypsin into the pancreatic duct.The serum levels of pancreatic amylase (AMY),high-sensitivity C-reactive protein (HCRP),tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10) were monitored at different time-points after the operation,and then all the animals were sacrificed on postoperative day 7.The pancreas specimens were collected for pathological examinations,and histologic score were assessed.The dogs which died during the study period were recorded,and the causes of death were analyzed.Results There was no significant difference between these three groups on the level of serum AMY、HCRP、TNF-α and IL-10 before the operation (P ＞ 0.05).We found that the level of serum AMY、HCRP、TNF-a and IL-10 in CG group were increased compared with SG group after the success of the moulding.The levels of TNF-a and HCRP were significantly higher in VG than in CG group (P =0.001),but the level of IL-10 was lower than CG group (P =0.023).However,there was no significant difference between VG and CG in terms of the levels of serum AMY (P =0.158).Pancreatic histologic score of SG was the lowest,and pancreatic histologic score of VG was significantly higher than CG (P =0.03).Two dogs in CG group died in the experiment,four dogs in VG group died,and no death occurred in SG group.Conclusions The result implies the vagus nerve as a regulating role in the early stage of ANP.Cutting vagus nerves could exacerbate the development of ANP. Key words: Vagotomy; Acute necrotizing pancreatitis (ANP) ; Cytokines