Effects of vagotomy during inflammation and cytokine production in hamster amebic liver abscess

Abstract

Event Abstract Back to Event Effects of vagotomy during inflammation and cytokine production in hamster amebic liver abscess Esperanza Sánchez A.1* 1 Universidad Autonoma de Aguascalientes, Morfología, Mexico The inflammatory response is modulated by its interactions with the nervous system. The vagus nerve and its neurotransmitter acetylcholine is the major parasympathetic innervation to chest and abdominopelvic viscera, Thus, through the alpha 7 nicotinic receptor on macrophages and other cells, inhibits NF-kB pathway reducing the secretion of pro-inflammatory cytokines. Entamoeba histolytica (Eh) in humans causes several diseases including amebic liver abscess (ALA) that is characterized by formation of inflammatory granulomatous lesions. In this context, we studied the role of vagotomy during induction of ALA in hamster, this was done by cutting the ventral vagal branches that innervate the liver and after 30 days was inoculated 350.000 Eh trophozoites (H1-IMSS strain) by intrahepatic via. Animals were sacrificed at 6, 12, 24h, 2, 4 and 7d post-inoculation, then, was analyzed NFKB activation in neutrophils and macrophages, and the presence of pro and anti-inflammatory cytokines+ cells by Immunohistochemical and immunofluorescence techniques. During ALA kinetics in parasimpatectomized hamsters was observed significant NFKB activation in neutrophils (10.43 ± 0.93) and macrophages (7.77 ± 0.15) and an increase of IL-1β+ (8.1 ± 1.0) and TNF-α+ cells (17.02 ± 1.77) compared to Sham and control groups. However, only IL8+ cells significantly increased at 24h post-inoculation in vagotomized animal. No changes were observed in IL10+ cells. Our results demonstrate that vagotomy in ALA model induces a pro-inflammatory response more durable due to increased activation of NFKB and therefore higher release of IL-1β and TNF-α cytokines. The vagus nerve is important in regulation of inflammatory process during ALA. Acknowledgements Quintanar-Stephano A, Campos-Esparza MR and Ventura-Juárez J.