Effects of vacuum UV and UVC radiation on dry Escherichia coli plasmid pUC19 II. Mutational specificity at the lacZ gene

Abstract

The mutational spectra at the lacZ gene, induced either by vacuum UV at 160 nm or UVC at 254 nm in vacuum-dried preparations of Escherichia coli plasmid pUC19 DNA, have been characterized from 72 E. coli-propagated mutants by DNA sequencing. In plasmids irradiated in vacuum, vacuum UV is five times more mutagenic than UVC. In the UV-induced mutants, base substitutions largely predominate, with GC → AT (G, guanine; C, cytosine; A, adenine; T, thymine) transitions being the most abundant type of base change for vacuum UV (61%) and UVC (47%). Most of the GC → AT transitions appear to occur at dipyrimidine sites, which are located at the non-transcribed DNA strand. Some, but not all, hot spots for GC → AT transitions are identical for vacuum UV and UVC. Frameshifts, resulting from a loss of the thymine residue, are specific for UVC (22%), and were not detected after treatent with vacuum UV. They occur predominantly at thymine runs of the transcribed DNA strand. Only a few deletions were detected following irradiation with vacuum UV (7.5%) and UVC (2%); however, their frequency is not enhanced compared with the spontaneous mutation spectrum. The data confirm the important role of base substitution mutations in UV-induced mutagenesis, which is not only valid for the UVC range, but extends towards the vacuum UV range.