Abstract
Scedosporium apiospermum and Lomentospora prolificans are important fungal species isolated from immunocompromised patients. Previous studies have demonstrated that these filamentous fungi exist as saprophytes in the soil and showed the highest minimum inhibitory concentration to several drugs. We aimed to examine how UVC affects the S. apiospermum and L. prolificans by investigating the role of UVC on growth, induction of apoptosis by ethidium bromide (EB)/acridine orange (AO) staining, and transcriptomic study of caspase recruitment domain family, member 9 (CARD-9) gene. Our studies showed that 15 minutes of exposure to UVC light effectively increased reduction in both organisms and caused changes in colony morphology, color, and hyphal growth pattern. After 15 min of UVC irradiation, apoptotic cells were quantitated by EB/AO staining, and the percentage of apoptosis was 96.06% in S. apiospermum and 28.30% in L. prolificans. CARD-9 gene expression results confirmed that apoptosis was induced in S. apiospermum and L. prolificans after UVC treatment and that S. apiospermum showed a higher expression of apoptosis signaling than L. prolificans. Our study explored the effects of UVC in the inactivation of S. apiospermum and L. prolificans. We hope that our data is useful to other researchers in future studies.
Highlights
Scedosporium apiospermum species complex is a group of emerging opportunistic fungal pathogens increasingly found in immunocompromised patients [1]
The remaining conidia suspension was centrifuged at 2,000 rpm for 5 min, and the pellet was resuspended in PBS and used apoptosis study by ethidium bromide (EB) and acridine orange (AO) staining, and transcriptomic study of the caspase recruitment domain family, member 9 (CARD-9) gene
In Thailand, S. apiospermum has been reportedly found in brain abscesses of neardrowning and renal transplant patients [8, 26] and L. prolificans has been reportedly found in a case of disseminated infection in a patient with acute myeloid leukemia with prolonged febrile neutropenia (Damronglerd et al 2014)
Summary
Scedosporium apiospermum species complex is a group of emerging opportunistic fungal pathogens increasingly found in immunocompromised patients [1]. S. apiospermum and Lomentospora prolificans (former name Scedosporium prolificans) are medically important fungal species that were isolated from patients with Scedosporium infections [2,3,4,5]. They demonstrated that UVC treatment carried out on day 0 and day 1 significantly reduced the fungal bioburden in the infected burns
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
