Abstract
Objective To investigate the effects of urinary trypsin inhibitor (UTI) on the proliferation,migration and invasion of SK-HEP-1 hepatocellular carcinoma cells in vitro.Methods Effects of UTI on SK-HEP-1 cells proliferation was evaluated by methyl thiazol tetrazolium (MTT) assay; Effects of migration and invasion by UTI on SK-HEP-1 cells was evaluated by Transwell assay ; The expression of urokinase type plasminogen activator (UPA) after UTI administration was evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blotting.Results UTI did not affect the proliferation of SK-HEP-1 cells ( P ＞ 0.05 ).UTI suppressed the migration and invasion of SK-HEP-1 cells dose-dependantly(P ＜ 0.05).UTI suppressed UPA expression of SK-HEP-1 on mRNA and protein level dose-dependantly(P ＜ 0.05),as compared with the control group.Conclusion UTI can suppress the migration and invasion of SK-HEP-1 hepatocellular cells.Decreasing the expression of UPA by UTI may be relevant to the mechanism of the migration and invasion suppression on SK-HEP-1 cells by UTI. Key words: Urinary trypsin inhibitor (UTI) ; Urokinase type plasminogen activator (UPA) ; Liver hepatocellular carcinoma SK-HEP-1 cells; Migration; Invasion
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