Year-end Sale % OFF 
Abstract
Objective To investigate the effects of urinary trypsin inhibitor (UTI) on the proliferation,migration and invasion of SK-HEP-1 hepatocellular carcinoma cells in vitro.Methods Effects of UTI on SK-HEP-1 cells proliferation was evaluated by methyl thiazol tetrazolium (MTT) assay; Effects of migration and invasion by UTI on SK-HEP-1 cells was evaluated by Transwell assay ; The expression of urokinase type plasminogen activator (UPA) after UTI administration was evaluated by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blotting.Results UTI did not affect the proliferation of SK-HEP-1 cells ( P ＞ 0.05 ).UTI suppressed the migration and invasion of SK-HEP-1 cells dose-dependantly(P ＜ 0.05).UTI suppressed UPA expression of SK-HEP-1 on mRNA and protein level dose-dependantly(P ＜ 0.05),as compared with the control group.Conclusion UTI can suppress the migration and invasion of SK-HEP-1 hepatocellular cells.Decreasing the expression of UPA by UTI may be relevant to the mechanism of the migration and invasion suppression on SK-HEP-1 cells by UTI. Key words: Urinary trypsin inhibitor (UTI) ; Urokinase type plasminogen activator (UPA) ; Liver hepatocellular carcinoma SK-HEP-1 cells; Migration; Invasion
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
