Effects of Urea Coated Hydroxyapatite Nanoparticles on Enzymatic and Nonenzymatic Antioxidants on Varieties of Brassica juncea L.

Abstract

In the present investigation, urea coated hydroxyapatite nanoparticles (UHANPs) were synthesized, and characterized by using X-ray diffraction, FESEM, and FTIR techniques. Then, their effects at different concentrations (250 ppm, 500 ppm, 750 ppm, 1000 ppm) were observed for various enzymatic and non-enzymatic antioxidants of two varieties of Brassica juncea L. i.e. GIRIRAJ, and NRCBH- 101. Those were also weighup with control plants and plants grown in treatments with the same concentrations of urea. Results revealed that synthesized nanoparticles were 23-32 nm in size, and for all the parameters, the antioxidants like NPT, Polychelatins were found to the higher at treatment with 1000 ppm of Urea when compared to others while minimum concentration of these with UHANP (500 ppm). However, SOD increases with higher concentration of UHANPs and urea while other enzymatic antioxidants like APX and catalase declines. However, SOD declined with higher concentration of urea. All the results were statistically compared.