Effects of upregulatioon of trophoblast cell-surface antigens 2 on migration, invasion and epithelial-mesenchymaltransition of human prostate cancer cells

Abstract

Objective To explore the effects of over-expression of trophoblast cell-surface antigens 2(TROP-2)gene on the migration and invasion of human prostate cancer cells and the action mechanism. Methods The TROP-2 gene was cloned into the eukaryotic expression vector pcDNA3. 1, and TROP-2 over-expression of eukaryotic gene expression plasmid was constructed. All PC-3 cells were divided into three groups: the control group(Con-A), empty vector control group(Con-B)and over-expression group TROP-2(pcDNA3. 1-TROP-2).After PC-3 cells in three groups were transfected with phosphate buffer(PBS), vector pcDNA3. 1, or pcDNA3. 1-TROP -2, the protein levels of TROP-2 were determined by Western blotting, respectively.The migration and invasion ability was evaluated by Transwell.The E-Cadherin and N-Cadherin protein expression in PC-3 cells was examined by Western blotting. Results TROP-2 gene eukaryotic expression vector was successfully constructed. The Western blotting showed that the expression levels of TROP-2 protein were greatly increased in PC-3 cancer cells transfected with pcDNA3. 1-TROP -2.The Transwell migration assay revealed that the number of migrating cells in Con-A,Con-B, and pcDNA3. 1-TROP-2 groups was 132. 6±2. 2, 130. 8±1. 8 and 189. 6±2. 6, respectively (P< 0. 01).The Transwell invasion assay indicated that the number of migrating cells in Con-A, Con-B, and pcDNA3. 1-TROP-2 groups was 118. 16±1. 96, 117. 52±1. 85 and 166. 38±1. 65, respectively(P< 0. 01).As Compared with the Con-A group, the E-cadherin protein expression was decreased,and the N-cadherin protein expression was increased in PC-3 cancer cells transfected with pcDNA3. 1-TROP-2. Conclusion TROP-2 overexpression can promote migration and invasion of human prostate cancer cells probably promoting the epithelial-mesenchymal transition. Key words: Trophoblast cell-surface antigens 2; Prostate carcinoma; Invasion; Epithelialmesenchymal transition