Effects of upregulatioon of Forkhead box protein M1 on migration, invasion and epithelial-mesenchymal transition of human ovarian cancer cell

Abstract

Objective To explore the effects and mechanism of over-expression of Forkhead box protein M1 (FoxM1) gene on the migration, and invasion and epithelial-mesenchymal transition (EMT) of human ovarian cancer cells. Methods FoxM1 over-expression of the virus vector was constructed. All HO-8910 cells were divided into three groups, the control group (Con-A), Lentiviral vector(LV) control group (Con-B) and over-expression group FoxM1. After three group HO-8910 cells was transfected with phosphate buffer (PBS), Lentiviral vector (LV), and LV-FoxM1, the protein levels of FoxM1 were determined by Western blotting, respectively. The migration and invasion ability was evaluated by wound healing assay and Transwell assay, respectively. The E-Cadherin and N-Cadherin of protein of cancer cells was examined by Western blotting assay. Results The results from Western blotting assay showed that the levels protein of FoxM1 were greatly increased in HO-8910 cancer cells transfected with LV-FoxM1. The results of the wound healing test showed that the migration distance of Con-A, Con-B, and LV-FoxM1 group was(156.80±2.26), (161.60±1.81) and (278.60±2.62) μm, respectively (P<0.01); The results of the Transwell invasion assay showed that the membrane cell number of Con-A, Con-B, and LV-FoxM1 group was (68.56±1.69) and (67.27±1.36) and (126.38±1.56), respectively (P<0.01); Compared with the Con-A, there were E-cadherin protein expression decreased; however, N-cadherin protein increased in HO-8910 cancer cells transfected with LV-FoxM1. Conclusion FoxM1 overexpression can promote human ovarian cancer cell migration and invasion, and its mechanism may be related to the promotion of epithelial-mesenchymal transition. Key words: Ovarian carcinoma; Forkhead box protein M1; Migration; Invasion; Epithelial-mesenchymal transition