Abstract
Background/AimsEmployment of regenerative properties of stem cells at the service of nerve repair has been initiated during recent decades. Effects of transplantation of undifferentiated cultured omental adipose-derived stem cells (uADSCs) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model. MethodsA 10-mm sciatic nerve defect was bridged using a silicone rubber chamber filled with uADSCs. In control group, the silicone conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied 4, 8, and 12wk after surgery. In sham-operated group, the sciatic nerve was only exposed and manipulated. ResultsFunctional study confirmed faster recovery of regenerated axons in uADSC-transplanted animals than in control group (P<0.05). Gastrocnemius muscle mass in uADSC-transplanted animals is found to be significantly more than that in control group. Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in uADSC-transplanted animals than in control group. In immunohistochemistry, location of reactions to S-100 in uADSC-transplanted animals was clearly more positive than that in control group. ConclusionuADSC transplantation could be considered as a readily accessible source of stromal cells that improve functional recovery of sciatic nerve.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
