Abstract
The influence of ultraviolet light and cyclophosphamide on the incorporation of tritium labeled thymidine in the presence and absence of hydroxyurea was studied in isolated rat liver cells and human leukocytes co-incubated with rat liver microsomes. In both cell systems, ultraviolet light as well as cyclophosphamide stimulated thymidine incorporation in the presence of hydroxyurea, and inhibited incorporation in the absence of hydroxyurea. This divergent effect on thymidine incorporation mediated by hydroxyurea can be used as a sensitive indication of genetic damage, taking advantage of the metabolizing capacity of isolated rat liver cells or microsomes.
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