Abstract
Chick blood infected with Plasmodium gallinaceum was subjected to ultrasound at a frequency of 20,000 cycles per second. The effects of intensity, duration of treatment, and dilution of the blood on the degree of disruption were studied. After gentle sonication the free parasites adhere closely to the free nuclei of disrupted erythrocytes. More thorough sonication yields a complex mixture of blood cells, erythrocyte nuclei, parasites, fragments in different states of degradation, malarial pigment, and artifacts of sonication. The ultrasound-resistant residuum is made up of the pigment, the artifacts, and a small amount of debris. The degree of treatment necessary to render the blood noninfective is affected by the number of parasites in the blood.
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