Abstract
The present work aims to test the bioeffects of ultrasounds on cyst formation in the crustacean Artemia salina. It is designed to identify which durations of ultrasonic exposure are capable of influencing hatching rates. The transducers used in the experiment were operated at resonant (0.23[Formula: see text]MHz and 4.7[Formula: see text]MHz) and non-resonant (0.5[Formula: see text]MHz and 1[Formula: see text]MHz) frequencies and the intensities of 3.25[Formula: see text]mW/cm2 and 13.63[Formula: see text]mW/cm2. The Rayleigh–Plesset theoretical framework was used to calculate the dynamics of shell-encapsulated cysts. The duration of ultrasound exposure was approximately 2[Formula: see text]min every 2[Formula: see text]h, repeated 6 times in the culture range of 1–15[Formula: see text]h. The results demonstrate that irradiation at an intensity of 13.63[Formula: see text]mW/cm2 and a driving frequency of 0.23[Formula: see text]MHz increased the number of hatched larvae by approximately 31.7%, compared with unexposed control samples. In conclusion, the theoretical model provided a useful means of predicting the most efficient frequency of sonication.
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