Abstract
The aims of this study were to investigate the influence of ubiquitin- conjugating enzyme E2C (UBE2C) on biological behavior of lung cancer cells. Using MTT, flow cytometry and invasion assays, we detected UBE2C expression and evaluated its biological properties in these cells, including effects on proliferation, the cell cycle profile and invasive capability. Compared with control cells, the UBE2C transfected cells demonstrated increased cellular proliferation (p<0.05). UBE2C transfected cells also had a lower percentage in G1 phase and a higher percentage in S phase (p<0.05). Importantly, the UBE2C transfected cells had a notable enhancement of cell numbers penetrating the basement membrane compared with the control group (p<0.05). Ectopic up- regulation UBE2C promoted the growth of lung cancer cells in vivo. Furthermore, we found UBE2C increased the expression of cyclin D1 and MMP-2. These results show UBE2C may represent a potential therapeutic target for lung cancer.
Highlights
Lung cancer is the leading cause for cancer-related deaths in the world
As the important role in regulating cell cycle, ubiquitin- conjugating enzyme E2C (UBE2C) is considered as an oncogene in a variety of cancers and its overexpression is associated with proliferation of tumors cells (Okamoto et al, 2003; Jiang et al, 2008; Troncone et al, 2009; Jiang et al, 2010)
Higher UBE2C expression was associated with a shorter postoperative survival time of non-small cell lung cancer (NSCLC) patients and may play an important role in NSCLC carcinogenesis (Zhao et al, 2012)
Summary
Lung cancer is the leading cause for cancer-related deaths in the world. The morbidity and mortality of the lung cancer patients in China were increased rapidly in the past decade (Jemal et al, 2008). As lung cancer cells infiltrate into surrounding tissue and metastasize to distant organs, the 5- year survival rate in lung cancer patients was very low (Rizzi et al, 1990). Some lifestyle related habits, such as smoking and alcohol consumption, are closely associated with lung cancer (Bhaskarapillai et al, 2012). The pathogenesis of lung cancer is inadequately understood. Elucidating the molecular mechanism that regulates the initiation and progression of lung cancer is vital to improve survival rate of patients
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