Abstract
: The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharides on the conversion of pig peripheral T lymphocytes. For this, 24 Sprague–Dawley rats were selected for the clinical trial and divided into groups B (blank), CK (cyclophosphamide inhibitory control), AP (angelica polysaccharide), and RIP (radix isatidis polysaccharide). Except for group B, other groups can induce the immunodeficiency by using cyclophosphamide. Rats of the AP and RIP groups were given gavage of 1 mL of AP and RIP. The blood was sampled from the eyeball on days 0, 7, 14, 21, 28, and 35, respectively, to determine immune cells, IgG and IgM of immunoglobulin, body weight, and spleen index.Results: The average content of AP and RIP was 51.27 and 14.8%, and the extraction rate was 75.23 and 60.94%. The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL−1 and 1.5 when the concentration of RIP was 125 μg mL−1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide. The two polysaccharides can regulate the rapid recovery of weight in immunosuppressed rats and increase the spleen index of immunosuppressed SD rats. The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine.
Highlights
Angelica, first contained in Agriculture God’s Canon of Materia Medica (Shennong Bencao Jing), is a common Chinese herbal medicine of the dried roots of Angelica [1]
The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL−1 and 1.5 when the concentration of radix isatidis polysaccharide (RIP) was 125 μg mL−1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide
The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine
Summary
First contained in Agriculture God’s Canon of Materia Medica (Shennong Bencao Jing), is a common Chinese herbal medicine of the dried roots of Angelica (the umbelliferae plants) [1]. Modern pharmacological studies show that AP can improve immunity and show antitumor, antiradiation, antioxidation, and antiaging effects [5,6,7]. The dried root of the isatis tinctoria (Cruciferous plant), recorded in Agriculture God’s Canon of Materia Medica, has the effect of clearing heat and detoxifying, cooling blood, and tonifying the pharynx [8]. Polysaccharide from traditional Chinese medicine can improve the immunity of animals [13], and it is reported to enhance the phagocytosis of the mono-macrophage system, activate the reticuloendothelium system, induce the proliferation of lymphocytes, recognize antigens, and improve the lethality of NK cells [14,15,16]. In a tumor-bearing mouse model, a sublethal dose of cyclophosphamide after treatment with 100 mg/kg Ganshan injection significantly reduced mortality and promoted recovery [17]
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