Effects of Two Different Dose Levels of Irradiation on Thioredoxin Reductase in the Kidney of Guinea Pigs

Yildiz Guney,Ummuhani Ozel Turkcu,Meltem Nalca Andrieu,Melike Erol,Ferhan Komurcu,Nedret Kilic,Ayse Hicsonmez,Aysen Dizman

doi:10.3844/ajisp.2006.58.60

Abstract

It is aimed to examine the state of the oxidant-antioxidant system in the liver of guinea pig caused by ionizing radiation at two different dose levels in the early period. The research was carried out on guinea pigs irradiated with the doses of 8 Gy (group 2) or 15 Gy (group 3) (single dose/whole body) in comparison with control group (group 1). The levels of thioredoxin reductase (TR) in the kidney was measured. The results obtained by non-irradiated and irradiated (8 Gy and 15 Gy) kidneys on guinea pigs indicated that; in 8-15 Gy irradiated animal kidneys TR activity elevated significantly (p<0.001) when compared to the controls, but there was no difference between 8 Gy and 15 Gy irradiated animal groups. The data have shown that gamma-irradiation results in significant increase in TR activity at two different dose levels.

Highlights

Radiation is known to produce various reactive oxygens species (ROS) in biological systems such as superoxide, hydrogen peroxide and hydroxyl radicals and various types of tissue damage due to free radical reactions[1]
The results obtained by non-irradiated and irradiated (8 Gy and 15 Gy) kidneys on guinea pigs presented in Fig. 1 indicated that; in 8-15 Gy irradiated animal kidneys thioredoxin reductase (TR) activity elevated significantly (p
The results of recent studies demonstrated that total body irradiation in rats causes tissue damage in kidneys, liver, lungs, colon and ileum[20]

Summary

Introduction

Radiation is known to produce various reactive oxygens species (ROS) in biological systems such as superoxide, hydrogen peroxide and hydroxyl radicals and various types of tissue damage due to free radical reactions[1]. The generation of ROS plays an important role in the pathogenesis of irradiation-induced tissue injury[2]. The biological effects of ionizing radiation result from energy deposition in irradiated cells and subsequent acute generation of short-lived ROS[8].

Results

Conclusion