Effects of tree species richness on fine root production varied with stand density and soil nutrients in subtropical forests

Abstract

Fine root production accounts for a large proportion of net primary production (NPP) in forest ecosystems that is highly responsive to environmental and biotic changes. The underlying mechanisms of the relationship between tree species richness and fine root production have not been fully examined. Here we hypothesized that: (i) the relationship between aboveground species richness and fine root production could be attributable to belowground spatial resource partitioning; (ii) either symmetrical or asymmetrical root proliferation to obtain nutrients leads to increased fine root production; and (iii) stand density affects the relationship between species richness and fine root production. We used an ingrowth core method to estimate fine root production coupled to molecular approaches for identifying the tree species of sampled fine roots within each ingrowth core. There was a significant and positive relationship between aboveground species richness and fine root production. The increase in fine root production might partially be attributed to asymmetrical root proliferation rather than belowground spatial resource partitioning. A piecewise structural equation model (SEM) linking stand density and soil nutrients revealed that both factors play dominant roles in mediating the effects of aboveground species richness on fine root production. Moreover, fine root production and relative abundance of fine root distribution within-layers both depended on the effects of aboveground species richness × stand density × soil phosphorus (P) interactions. Therefore, soil P concentration and stand density partially explained the positive aboveground species richness–fine root production relationship.