Abstract
Objective To observe the effect of transplantation of bone marrow mesenchymal stem cells (BMSCs) transfected by insulin-like growth factor-1 ( IGF-1 ) gene on fracture healing of diabetic rats.Methods Fifty male Wistar rats were divided randomly into control group and transfection group.Streptozotocin was injected into celiac to induce diabetes model and all suffered right tibia fracture.Ad-IGF-1 transfected rat BMSCs under high glucose condition in vitro. After transplantation of BMSCs transfected by IGF-1,X-ray photographs were taken at 1,2,3,4,6 weeks,and the callus was subjected to hematoxylin and eosin (HE) staining.The levels of IGF-1 of the callus and serum were detected by immunohistochemical method and enzyme linked immunosorbent assay (ELISA) respectively. Results There were significant differences in the expression of the IGF-1 gene between the two groups.At 4 weeks,the gray scales of IGF-1 were 103 ±5 in transfection group and 109 ±4 in control group (P ＜0.05).The serum concentrations of IGF-1 at 4 weeks were (668.80 ±8.07) ng/L in transfection group and (569.20 ±9.31 ) ng/L in control group (P ＜0.01 ).Conclusion The transplantation of BMSCs transfected by IGF-1gene can promote the fracture healing of diabetic rats. Key words: Bone marrow mesenchymal stem cells; Insulin-like growth factor-1 ; Diabetes; Fracture; Transfect
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